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Production of structured triacylglycerols rich in palmitic acid at sn-2 position and oleic acid at sn-1,3 positions as human milk fat substitutes by enzymatic acidolysis

脂肪酶 弥黑 化学 油酸 棕榈酸 米根霉 色谱法 单酰甘油脂肪酶 生物化学 脂肪酸 甘油三酯酶 有机化学 内大麻素系统 发酵 受体
作者
Luis Esteban,M.J. Jiménez,Estrella Hita,P. González,Lorena Martín,Alfonso Robles
出处
期刊:Biochemical Engineering Journal [Elsevier]
卷期号:54 (1): 62-69 被引量:57
标识
DOI:10.1016/j.bej.2011.01.009
摘要

This paper studies the synthesis of structured triacylglycerols (STAGs) of type oleic acid–palmitic acid–oleic acid (OA–PA–OA or OPO) by acidolysis of PA enriched triacylglycerols (TAGs) (79.2% PA, 74.5% PA at sn-2 position) and several OA-rich free fatty acid (FFA) fractions. The PA located at sn-1,3 positions was replaced by OA, maintaining the PA at sn-2 position; for this purpose several sn-1,3 specific lipases (lipase DF from Rhizopus oryzae, Palatase 20000L from Mucor miehei, Lipozyme RM IM from Rhizomucor miehei, Lipozyme TL IM from Thermomyces lanuginosus and lipase QLC from Alcaligenes sp.) were tested in experiments carried out in presence of solvent at 37 °C. Lipase DF immobilized on Accurel MP1000 was selected because this lipase gave a high incorporation of OA at the extreme positions of TAGs (50.4%) in short reaction times (1 h) or IOT (intensity of treatment, lipase amount × reaction time/TAG amount) of only 0.4 g lipase × h/g TAG, maintaining a high content of PA at sn-2 position (68.6%). Using this lipase, the influence of FFA/TAG molar ratio and purity of OA-rich FFAs were studied. With a 6:1 FFA/TAG molar ratio and 90% OA it was possible to obtain STAGs with 67.2% OA at sn-1,3 positions and 67.8% PA at sn-2 position (57.2% of total PA was located at sn-2 position). Next, the synthesis of OPO STAGs was studied in solvent-free media at 50 °C. The best results were also attained with a 6:1 FFA/TAG molar ratio, 90% OA and using an IOT of 1.9 g lipase × h/g TAG. In these conditions it was possible to obtain STAGs with identical composition to the previously obtained with solvent. These STAGs were purified by two procedures which differed in that solvent was or was not used to separate STAGs and FFAs. Both with and without hexane, STAGs were obtained with approximately 99% purity and over 96% recovery yields.
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