透明质酸酶
透明质酸
氯化十六烷基吡啶
色谱法
化学
琼脂糖
印版阅读器
试剂
微量滴定板
生物化学
酶
生物
荧光
有机化学
肺表面活性物质
遗传学
物理
量子力学
作者
J S Tung,George E. Mark,Gregory Hollis
标识
DOI:10.1006/abio.1994.1560
摘要
A sensitive, high-throughput assay has been developed which measures hyaluronidase activity in a microplate format. In this assay, hyaluronic acid is suspended in agarose in a microtiter well, the plate is incubated with the hyaluronidase solution, and the undigested hyaluronic acid is precipitated with cetylpyridinium chloride. The precipitate blocks light transmittance and therefore an increase in the visible light transmitted correlates with the amount of digested hyaluronic acid. Using this assay, as little as 0.05 units of hyaluronidase activity can be detected. The assay is highly reproducible and can be run with commercially available reagents. Hyaluronidase activity is easy to quantitate using a microplate reader and this format allows large numbers of samples to be assayed.
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