亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Solution-state 2D NMR of ball-milled plant cell wall gels in DMSO-d6/pyridine-d5

木质素 化学 细胞壁 分馏 裸子植物 碳-13核磁共振 质子核磁共振 核磁共振谱数据库 核磁共振波谱 吡啶 有机化学 植物 谱线 生物 天文 物理 生物化学
作者
Hoon Kim,John Ralph
出处
期刊:Organic and Biomolecular Chemistry [Royal Society of Chemistry]
卷期号:8 (3): 576-591 被引量:596
标识
DOI:10.1039/b916070a
摘要

NMR fingerprinting of the components of finely divided plant cell walls swelled in DMSO has been recently described. Cell wall gels, produced directly in the NMR tube with perdeutero-dimethylsulfoxide, allowed the acquisition of well resolved/dispersed 2D 13C–1H correlated solution-state NMR spectra of the entire array of wall polymers, without the need for component fractionation. That is, without actual solubilization, and without apparent structural modification beyond that inflicted by the ball milling and ultrasonication steps, satisfactorily interpretable spectra can be acquired that reveal compositional and structural details regarding the polysaccharide and lignin components in the wall. Here, the profiling method has been improved by using a mixture of perdeuterated DMSO and pyridine (4 : 1, v/v). Adding pyridine provided not only easier sample handling because of the better mobility compared to the DMSO-d6-only system but also considerably elevated intensities and improved resolution of the NMR spectra due to the enhanced swelling of the cell walls. This modification therefore provides a more rapid method for comparative structural evaluation of plant cell walls than is currently available. We examined loblolly pine (Pinus taeda, a gymnosperm), aspen (Populus tremuloides, an angiosperm), kenaf (Hibiscus cannabinus, an herbaceous plant), and corn (Zea mays L., a grass, i.e., from the Poaceae family). In principle, lignin composition (notably, the syringyl : guaiacyl : p-hydroxyphenyl ratio) can be quantified without the need for lignin isolation. Correlations for p-coumarate units in the corn sample are readily seen, and a variety of the ferulate correlations are also well resolved; ferulates are important components responsible for cell wall cross-linking in grasses. Polysaccharide anomeric correlations were tentatively assigned for each plant sample based on standard samples and various literature data. With the new potential for chemometric analysis using the 2D NMR fingerprint, this gel-state method may provide the basis for an attractive approach to providing a secondary screen for selecting biomass lines and for optimizing biomass processing and conversion efficiencies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
老石完成签到 ,获得积分10
5秒前
15秒前
CipherSage应助科研通管家采纳,获得10
1分钟前
彭于晏应助科研通管家采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
火星上向珊完成签到,获得积分10
1分钟前
1分钟前
wdxx发布了新的文献求助30
1分钟前
liufan完成签到 ,获得积分10
2分钟前
lmplzzp完成签到,获得积分10
2分钟前
橙子味的邱憨憨完成签到 ,获得积分10
2分钟前
杪夏二八完成签到 ,获得积分10
2分钟前
wdxx完成签到,获得积分10
2分钟前
649981108发布了新的文献求助10
3分钟前
3分钟前
649981108完成签到,获得积分10
3分钟前
3分钟前
研友_892kOL完成签到,获得积分10
3分钟前
脑洞疼应助李小猫采纳,获得10
3分钟前
4分钟前
李小猫完成签到,获得积分10
4分钟前
4分钟前
李小猫发布了新的文献求助10
4分钟前
4分钟前
4分钟前
5分钟前
6分钟前
7分钟前
Tiger完成签到,获得积分10
7分钟前
7分钟前
7分钟前
Ma发布了新的文献求助10
7分钟前
7分钟前
7分钟前
科研通AI5应助Ma采纳,获得10
7分钟前
彼岸花开发布了新的文献求助10
8分钟前
8分钟前
易四夕发布了新的文献求助10
8分钟前
9分钟前
科研通AI2S应助科研通管家采纳,获得10
9分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968504
求助须知:如何正确求助?哪些是违规求助? 3513278
关于积分的说明 11167214
捐赠科研通 3248660
什么是DOI,文献DOI怎么找? 1794386
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804638