Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex

高尔基体 生物 布雷菲尔德A 免疫沉淀 内质网 细胞生物学 膜蛋白 高尔基膜 生物化学 分泌途径 胞浆 分子生物学 基因
作者
Heike B. Eberle,Ramón Serrano,Joachim Füllekrug,Andreas Schlösser,Wolf D. Lehmann,Friedrich Lottspeich,Dora V. Kaloyanova,Felix Wieland,J. Bernd Helms
出处
期刊:Journal of Cell Science [The Company of Biologists]
卷期号:115 (4): 827-838 被引量:100
标识
DOI:10.1242/jcs.115.4.827
摘要

Group 1 of plant pathogenesis-related proteins (PR-1) and a variety of related mammalian proteins constitute a superfamily of proteins that share structural similarities. Little is known about their function, but all the family members identified to date are co-translationally translocated to the lumen of the endoplasmic reticulum and are secreted as soluble proteins or are targeted to vacuoles. Here we report the identification of a novel family member that localizes to the cytosolic site of the endomembrane system in mammalian cells. After detergent solubilization of isolated Golgi membranes, a 17 kDa protein was found associated with a low-density detergent-insoluble fraction. The amino-acid sequence, determined by microsequencing and molecular cloning, revealed a significant homology with the superfamily of PR-1 proteins. Golgi-associated PR-1 protein (GAPR-1) showed a brefeldin-A-sensitive Golgi localization in immunofluorescence. Interestingly,the protein remained associated with the microdomain fraction in the presence of Brefeldin A. By mass spectrometry, GAPR-1 was shown to be myristoylated. Immunoprecipitation of GAPR- 1 from Golgi membranes resulted in the coimmunoprecipitation of caveolin-1, indicating a direct interaction between these two proteins. Myristoylation, together with protein-protein or electrostatic interactions at physiological pH owing to the highly basic pI of GAPR-1 (pI 9.4) could explain the strong membrane association of GAPR-1. Tissue screening revealed that GAPR-1 is not detectably expressed in liver,heart or adrenal glands. High expression was found in monocytes, leukocytes,lung, spleen and embryonic tissue. Consistent with the involvement of PR-1 proteins in the plant immune system, these data could indicate that GAPR-1 is involved in the immune system.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
石勒苏益格完成签到,获得积分10
1秒前
赵先森完成签到 ,获得积分10
2秒前
涛1完成签到 ,获得积分10
4秒前
SAINT完成签到 ,获得积分10
11秒前
lbyscu完成签到 ,获得积分10
11秒前
粥粥完成签到 ,获得积分10
12秒前
JACK完成签到,获得积分10
14秒前
Wang发布了新的文献求助10
15秒前
rice0601完成签到,获得积分10
15秒前
mawenting完成签到 ,获得积分10
16秒前
lpx43完成签到,获得积分10
17秒前
一针超人完成签到 ,获得积分10
18秒前
Jasper应助Wang采纳,获得10
23秒前
55555发布了新的文献求助10
26秒前
吴宵完成签到,获得积分0
29秒前
31秒前
飞哥完成签到 ,获得积分10
39秒前
胡星星完成签到 ,获得积分10
39秒前
collapsar1完成签到,获得积分10
40秒前
科研通AI2S应助55555采纳,获得10
44秒前
雨洋完成签到,获得积分10
45秒前
WLY完成签到 ,获得积分10
45秒前
葶ting完成签到 ,获得积分10
45秒前
nkmenghan完成签到,获得积分10
51秒前
Hyacinth完成签到 ,获得积分10
54秒前
耀学菜菜完成签到,获得积分10
58秒前
陈少华完成签到 ,获得积分10
1分钟前
江三村完成签到 ,获得积分10
1分钟前
c_123完成签到 ,获得积分10
1分钟前
柳树完成签到,获得积分10
1分钟前
1分钟前
跪斗丶完成签到 ,获得积分10
1分钟前
Danielle完成签到,获得积分10
1分钟前
Yolo完成签到 ,获得积分10
1分钟前
好名字完成签到,获得积分10
1分钟前
是述不是沭完成签到,获得积分10
1分钟前
1分钟前
Joy完成签到 ,获得积分10
1分钟前
zodiac完成签到,获得积分10
1分钟前
xshlzwyyh完成签到,获得积分10
1分钟前
高分求助中
The late Devonian Standard Conodont Zonation 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3244791
求助须知:如何正确求助?哪些是违规求助? 2888434
关于积分的说明 8252939
捐赠科研通 2556941
什么是DOI,文献DOI怎么找? 1385522
科研通“疑难数据库(出版商)”最低求助积分说明 650176
邀请新用户注册赠送积分活动 626303