The internal control role of ribosomal protein S7 in the defense of Anopheles dirus against Plasmodium infection

OBJECTIVE To investigate the role of ribosomal protein S7 (rpS7) in the defense of Anopheles dirus against infection. METHODS rpS7 was amplified from Anopheles dirus hemocytes with degenerated primers designed according to the conservative region of S7, rpS7 was then cloned using T/A cloning kit and the inserted fragment was sequenced. The difference of the transcript abundance of rpS7 from Anopheles dirus hemocyte among non-blood-fed (N), normal-blood-fed (B) and Plasmodium yoelii infected groups (I) was also analyzed by RT-PCR and gel scanning system at d1, d2, d3, d4, d7 and d11 after blood feeding. RESULTS There is no significant difference of rpS7 signal between the three groups. CONCLUSION Anopheles dirus S7 can be used as an internal control to study the role of Anopheles dirus related immune factors in Plasmodium infection.