DNA Preparation of Toxigenic Aspergillus and PCR Detection of aflR Toxin Gene

This paper discusses the modified CTAB method for DNA extraction of toxigenic Aspergillus and compares it with the kit extraction method. The modified CTAB method successfully extracted DNA of toxigenic Aspergillus. The concentration and purity reached the standard of PCR amplification. Two pairs of primers were designed based on the aflR gene for aflatoxin biosynthesis regulation, and the toxigenic Aspergillus aflR gene was identified by using PCR amplification. The results show that the aflR gene is contained in the genome of Aspergillus flavus, Aspergillus parasitic and Aspergillus tamarii, Aspergillus fumigates and Aspergillus versicolor are not detected, realizing the purpose for identifying aflatoxin producing strains. This method provides a reference for rapid detection of toxigenic fungi.