Toxicity of Co-contamination with Bensulfuron Methyl and Cadmium to Cell and DNA of Rice

To investigate the effects of co-contamination of bensulfuron methyl and cadmium on rice at cellular and molecular levels, transmission electron microscope (TEM), scanning electron microscope (SEM) and comet assay were used. Two rice cultivars, Yue Xiangzhan and Feng Meizhan, were employed as the indicator plants. TEM and SEM were used to assess the toxicity of Cd and BSM to rice at the cellular level. The result indicated that BSM and Cd could destroy cellular structure, disrupt epidermic cells and result in nucleolus resolution. The comet assay was used to investigate the toxicity of Cd and BSM to Feng Meizhan at molecular level by analyzing the photograph with CASP. The result indicated that there was a dose dependent increase in DNA damages with the increasing concentrations of Cd in the solution. When 0.1 mg·L-1 BSM was added in the solution with 10 mg·L-1 Cd, the Olive TM was decreased, indicating the toxicity of Cd to the rice DNA could be relieved when the 0.1 mg·L-1 BSM was supplied.