内生
血脑屏障
细胞生物学
内吞循环
薄壁组织
细胞内
免疫球蛋白G
化学
抗体
生物
内吞作用
免疫学
细胞
生物化学
中枢神经系统
内分泌学
植物
作者
Roberto Villaseñor,Laurence Ozmen,Nadia Messaddeq,Fiona Grüninger,Hansruedi Loetscher,Annika Keller,Christer Betsholtz,Per‐Ola Freskgård,Ludovic Collin
摘要
Abstract The Blood-Brain Barrier (BBB) restricts access of large molecules to the brain. The low endocytic activity of brain endothelial cells (BECs) is believed to limit delivery of immunoglobulins (IgG) to the brain parenchyma. Here, we report that endogenous mouse IgG are localized within intracellular vesicles at steady state in BECs in vivo . Using high-resolution quantitative microscopy, we found a fraction of endocytosed IgG in lysosomes. We observed that loss of pericytes (key components of the BBB) in pdgf-b ret/ret mice affects the intracellular distribution of endogenous mouse IgG in BECs. In these mice, endogenous IgG was not detected within lysosomes but instead accumulate at the basement membrane and brain parenchyma. Such IgG accumulation could be due to reduced lysosomal clearance and increased sorting to the abluminal membrane of BECs. Our results suggest that, in addition to low uptake from circulation, IgG lysosomal degradation may be a downstream mechanism by which BECs further restrict IgG access to the brain.
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