病毒学
病毒载量
卡波西肉瘤相关疱疹病毒
生物
爱泼斯坦-巴尔病毒
背景(考古学)
实时聚合酶链反应
病毒
聚合酶链反应
伽马赫氏病毒亚科
数字聚合酶链反应
疱疹病毒科
病毒性疾病
基因
遗传学
古生物学
作者
Patricio Cano,Tischan Seltzer,Jedediah Seltzer,Alice Peng,Justin T. Landis,Linda Pluta,Dirk P. Dittmer
摘要
ABSTRACT “ When you can measure what you are speaking about, and express it in numbers, you know something about it .” is a famous quote attributed to Lord Kelvin. This sentiment puts viral load measurements at the center of virology. Viral load, or more precisely, DNA copy number measurements, are also used to follow infections with human herpesviruses, such as Kaposi sarcoma herpesvirus (KSHV) and Epstein–Barr Virus (EBV). EBV and KSHV are associated with human cancers, and determining their DNA copy numbers in the context of cancer prediction and progression on therapy is of fundamental scientific and translational interest. Yet, there is no generally accepted assay for KSHV DNA quantitation, and KSHV viral load is not used in clinical decision‐making. Here, we review the history of KSHV DNA detection assays, explore factors that affect sensitivity and specificity, and describe an automated, high‐throughput, real‐time quantitative polymerase chain reaction (PCR) assay for KSHV and EBV. In conjunction with a digital PCR assay using the same primer/probe combination, we describe how to determine the absolute KSHV genome copy numbers in plasma, peripheral blood mononuclear cells, saliva, and other easily accessible body fluids.
科研通智能强力驱动
Strongly Powered by AbleSci AI