Production of Reactive Oxygen Species during Redox Manipulation and Its Potential Impacts on Activated Sludge Wastewater Treatment Processes

Reactive oxygen species (ROS) are ubiquitous in redox-fluctuating environments, exerting profound impacts on biogeochemical cycles. However, whether ROS can be generated during redox manipulation in activated sludge wastewater treatment processes (AS-WTPs) and the underlying impacts remain largely unknown. This study demonstrates that ROS production is ubiquitous in AS-WTPs due to redox manipulation and that the frequency and capacity of ROS production depend on the operating modes. The anaerobic/oxic continuous-flow reactor showed persistent ROS generation (0.8-2.1 μM of instantaneous H