To improve the service reliability and fatigue life of truck-mounted cranes, this paper conducted dynamic analysis and fatigue life evaluation of the crane. The multi-body dynamics model of the crane was established, and the dynamic characteristics of the crane were analyzed. The load-time history of the crane is obtained. Based on the load time history of the crane, the finite element model of the crane is built, and the dynamic stress and maximum stress location of the crane are obtained. Following this, the fatigue life of the crane structure under sequential working conditions was analyzed using the nominal stress method and the load spectrum. The results could support the reliability improvement of the crane's service reliability.