Sphingobium cyanobacteriorum sp. nov., isolated from fresh water

A novel Gram-stain-negative, yellow-pigmented, short rod-shaped bacterial strain, HBC34 T , was isolated from a freshwater sample collected from Daechung Reservoir, Republic of Korea. The results of 16S rRNA gene sequence analysis indicated that HBC34 T was affiliated with the genus Sphingobium and shared the highest sequence similarity to the type strains of Sphingobium vermicomposti (98.01 %), Sphingobium psychrophilum (97.87 %) and Sphingobium rhizovicinum (97.59 %). The average nucleotide identity (ANI) and digital DNA–DNA hybridisation (dDDH) values between HBC34 T and species of the genus Sphingobium with validly published names were below 84.01 and 28.1 %, respectively. These values were lower than the accepted species-delineation thresholds, supporting its recognition as representing a novel species of the genus Sphingobium . The major fatty acids (>10 % of the total fatty acids) were identified as summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ) and summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ). The main polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two phospholipids and two unidentified polar lipids. The respiratory quinone was Q-10. The genomic DNA G+C content of HBC34 T was 64.04 %. The polyphasic evidence supports the classification of HBC34 T as the type strain of a novel species of the genus Sphingobium, for which the name Sphingobium cyanobacteriorum sp. nov is proposed. The type strain is HBC34 T (= KCTC 8002 T = LMG 33140 T ).