特应性皮炎
发病机制
银屑病
骨膜炎
生物标志物
医学
免疫组织化学
免疫印迹
免疫学
皮肤病科
病理
内科学
生物
基因
遗传学
细胞外基质
作者
Tanel Traks,Paula Reemann,Kattri‐Liis Eskla,Aigar Ottas,Toomas Jagomäe,Rasmus Liira,Liis Ilves,Viljar Jaks,Liisi Raam,Kristi Abram,Külli Kingo
摘要
Abstract Common characteristics in the pathogenesis of psoriasis (PS) and atopic dermatitis (AD) have been presumed, but only a few studies have clearly supported this. The current aim was to find possible similarities and differences in protein expression patterns between these two major chronic inflammatory skin diseases. High‐throughput tandem mass spectrometry proteomic analysis was performed using full thickness skin samples from adult PS patients, AD patients and healthy subjects. We detected a combined total of 3045 proteins in the three study groups. According to principal component analysis, there was significant overlap between the proteomic profiles of PS and AD, and both clearly differed from that of healthy skin. The following validation of selected proteins with western blot analysis showed similar tendencies in expression levels and produced statistically significant results. The expression of periostin (POSTN) was consistently high in AD and very low or undetectable in PS (5% FDR corrected p < 0.001), suggesting POSTN as a potential biomarker to distinguish these diseases. Immunohistochemistry further confirmed higher POSTN expression in AD compared to PS skin. Overall, our findings support the concept that these two chronic skin diseases might share considerably more common mechanisms in pathogenesis than has been suspected thus far.
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