环介导等温扩增
脑膜炎奈瑟菌
微流控
脑膜炎
纳米技术
生物传感器
计算生物学
医学
生物
计算机科学
细菌
材料科学
DNA
遗传学
精神科
作者
Hamed Tavakoli,Elisabeth Hirth,Man Luo,Sanjay Sharma,Maowei Dou,Delfina C. Domínguez,Xiujun Li
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:22 (23): 4693-4704
被引量:12
摘要
Bacterial meningitis, an infection of the membranes (meninges) and cerebrospinal fluid (CSF) surrounding the brain and spinal cord, is one of the major causes of death and disability worldwide. Higher case-fatality rates and short survival times have been reported in developing countries. Hence, a quick, straightforward, and low-cost approach is in great demand for the diagnosis of meningitis. In this research, a microfluidic fully paper-based analytical device (μFPAD) integrated with loop-mediated isothermal amplification (LAMP) and ssDNA-functionalized graphene oxide (GO) nano-biosensors was developed for the first time for a simple, rapid, low-cost, and quantitative detection of the main meningitis-causing bacteria, Neisseria meningitidis (N. meningitidis). The results can be successfully read within 1 hour with the limit of detection (LOD) of 6 DNA copies per detection zone. This paper device also offers versatile functions by providing a qualitative diagnostic analysis (i.e., a yes or no answer), confirmatory testing, and quantitative analysis. These features make the presented μFPAD capable of a simple, highly sensitive, and specific diagnosis of N. meningitis. Furthermore, this microfluidic approach has great potential in the rapid detection of a wide variety of different other pathogens in low-resource settings.
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