氨基酸
细菌
儿茶酚
胶粘剂
氨基酸残基
材料科学
生物化学
纳米技术
化学
组合化学
生物
肽序列
遗传学
图层(电子)
基因
作者
Mengxi Zhang,Yuda Chen,Anna Chung,Shudan Yang,Chi Hun Choi,Sophie S. Zhang,Yimo Han,Han Xiao
标识
DOI:10.1002/smtd.202400230
摘要
Abstract 3,4‐Dihydroxy‐L‐phenylalanine (DOPA) serves as a post‐translational modification amino acid present in mussel foot proteins. Mussels exploit the exceptional adhesive properties of DOPA to adhere to a wide range of surfaces. This study presents the development of sticky proteins and bacteria through the site‐specific incorporation of DOPA using Genetic Code Expansion Technology. Through the optimization of the DOPA incorporation system, proteins containing DOPA demonstrate significantly improved binding abilities to various organic and metallic materials. The material‐binding capabilities of DOPA to combat different types of biofoulings are harnessed by integrating it into intrinsically disordered proteins. Beyond the creation of adhesive proteins for anti‐biofouling purposes, this highly efficient DOPA incorporation system is also applied to engineer adhesive bacteria, resulting in a remarkable increase in their binding capability to diverse materials including 400 folds of improvement to polyethylene terephthalate (PET). This substantial enhancement in PET binding of these bacteria has allowed to develop a unique approach for PET degradation, showcasing the innovative application of Genetic Code Expansion in cell engineering.
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