Responses of juvenile blacklegged ticks (Acari: Ixodidae) to hosts of varying quality

Abstract Blacklegged ticks (Ixodes scapularis) are the most medically and economically important vectors in North America. Each of their 3 life stages requires a blood meal from one of many potential host species, during which they can acquire or transmit pathogens. Host species, however, vary tremendously in their quality for ticks, as measured by differences in feeding and molting success. There should be clear fitness benefits for ticks that preferentially feed upon high-quality hosts (e.g., white-footed mice, Peromyscus leucopus), or at least avoid feeding on very low-quality hosts (e.g., Virginia opossums, Didelphis virginiana). Indeed, laboratory experiments have found some evidence of host preferences in I. scapularis; but these involve presenting ticks with hosts simultaneously and measuring movement towards hosts on a horizontal plane. In nature, however, host-seeking ticks encounter hosts sequentially and their movements are principally in a vertical plane. Here, we present the results of a study in which we measured the vertical movements of host-seeking juvenile blacklegged ticks before and after a host (P. leucopus, Tamias striatus, Sciurus carolinensis, or D. virginiana) was present, and whether the strength of their responses varies with host quality. We found ticks did not measurably alter the speed of their vertical movement in the presence of any hosts, regardless of host quality. Both larvae and nymphs quested slightly higher in the presence of hosts, but this did not vary by host species. These results call into question the existence of active host preferences, at least in this stage of the host-seeking process.