Mangiferin reduces uric acid via regulation of amino acid and lipid metabolism

Mangiferin, a functional compound extracted from edible plants, has been shown to exhibit favorable uric acid-lowering properties. However, the underlying molecular mechanisms still require further investigation. In this study, we utilized a rat model of hyperuricemia to assess the hypouricemic effect of mangiferin and explore its potential mechanism based on UHPLC-Q-Exactive Orbitrap mass spectrometry. Untargeted metabolomics analysis revealed 19 differential metabolites significantly altered in serum of hyperuricemic rats through principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), which were primarily related to amino acid metabolism and lipid metabolism. Among these differential metabolites, the levels of alpha-methylstyrene, indan-1-ol, and 4-D-hydroxysphinganine significantly increased, while the levels of L-leucine, 2-phenylacetamide, hippuric acid, benzoic acid, isoquinoline, phenylpyruvate, glycerone phosphate, N, N-diethylphenylacetamide and corticosterone significantly decreased in model rats. After mangiferin intervention, 14 metabolites were reversed through modulation of metabolic pathways. Our findings suggest that mangiferin may serve as a useful potential adjuvant in reducing uric acid effects.