生物转化
羟基烷酸
木质素
化学
恶臭假单胞菌
儿茶酚
发酵
同种类的
生物转化
有机化学
生物降解
食品科学
酶
细菌
生物
物理
遗传学
热力学
作者
Zhi‐Min Zhao,Xianzhi Meng,Yunqiao Pu,Mi Li,Yibing Li,Yihan Zhang,Fang Chen,Arthur J. Ragauskas
出处
期刊:Biomacromolecules
[American Chemical Society]
日期:2023-08-09
卷期号:24 (9): 3996-4004
被引量:5
标识
DOI:10.1021/acs.biomac.3c00288
摘要
The bioconversion of homogeneous linear catechyl lignin (C-lignin) to polyhydroxyalkanoates (PHA) was examined for the first time in this study. C-lignins from vanilla, euphorbia, and candlenut seed coats (denoted as C1, C2, and C3, respectively) varied in their molecular structures, which showed different molecular weight distributions, etherification degrees, and contents of hydroxyl groups. A notable amount of nonetherified catechol units existed within C1 and C2 lignins, and these catechol units were consumed during fermentation. These results suggested that the nonetherified catechol structure was readily converted by Pseudomonas putida KT2440. Since the weight-average molecular weight of C2 raw lignin was 26.7% lower than that of C1, the bioconversion performance of C2 lignin was more outstanding. The P. putida KT2440 cell amount reached the maximum of 9.3 × 107 CFU/mL in the C2 medium, which was 37.9 and 82.4% higher than that in the C1 and C3 medium, respectively. Accordingly, PHA concentration reached 137 mg/L within the C2 medium, which was 41.2 and 149.1% higher than the C1 and C3 medium, respectively. Overall, C-lignin, with a nonetherified catechol structure and low molecular weight, benefits its microbial conversion significantly.
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