Chemically-induced epileptic seizures in zebrafish: A systematic review

癫痫 荟萃分析 斯科普斯 科学网 斑马鱼 样本量测定 医学 心理学 出版偏见 纳入和排除标准 梅德林 内科学 生物 精神科 病理 替代医学 统计 基因 生物化学 数学
作者
Rafael Chitolina,Matheus Gallas‐Lopes,Carlos G. Reis,Radharani Benvenutti,Thailana Stahlhofer-Buss,María Elisa Calcagnotto,Ana Paula Herrmann,Ângelo Piato
出处
期刊:Epilepsy Research [Elsevier BV]
卷期号:197: 107236-107236 被引量:3
标识
DOI:10.1016/j.eplepsyres.2023.107236
摘要

The use of zebrafish as a model organism is gaining evidence in the field of epilepsy as it may help to understand the mechanisms underlying epileptic seizures. As zebrafish assays became popular, the heterogeneity between protocols increased, making it hard to choose a standard protocol to conduct research while also impairing the comparison of results between studies. We conducted a systematic review to comprehensively profile the chemically-induced seizure models in zebrafish. Literature searches were performed in PubMed, Scopus, and Web of Science, followed by a two-step screening process based on inclusion/exclusion criteria. Qualitative data were extracted, and a sample of 100 studies was randomly selected for risk of bias assessment. Out of the 1058 studies identified after removing duplicates, 201 met the inclusion criteria. We found that the most common chemoconvulsants used in the reviewed studies were pentylenetetrazole (n = 180), kainic acid (n = 11), and pilocarpine (n = 10), which increase seizure severity in a dose-dependent manner. The main outcomes assessed were seizure scores and locomotion. Significant variability between the protocols was observed for administration route, duration of exposure, and dose/concentration. Of the studies subjected to risk of bias assessment, most were rated as low risk of bias for selective reporting (94%), baseline characteristics of the animals (67%), and blinded outcome assessment (54%). Randomization procedures and incomplete data were rated unclear in 81% and 68% of the studies, respectively. None of the studies reported the sample size calculation. Overall, these findings underscore the need for improved methodological and reporting practices to enhance the reproducibility and reliability of zebrafish models for studying epilepsy. Our study offers a comprehensive overview of the current state of chemically-induced seizure models in zebrafish, highlighting the common chemoconvulsants used and the variability in protocol parameters. This may be particularly valuable to researchers interested in understanding the underlying mechanisms of epileptic seizures and screening potential drug candidates in zebrafish models.
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