适体
荧光
检出限
荧光染料
化学
线性范围
生物物理学
纳米技术
分子生物学
材料科学
色谱法
实时聚合酶链反应
生物
生物化学
光学
基因
物理
作者
Hamed Zahraee,Zahra Khoshbin,Mohammad Ramezani,Mona Alibolandi,Khalil Abnous,Seyed Mohammad Taghdisi
标识
DOI:10.1016/j.saa.2022.122305
摘要
In this study, a sensitive fluorescent method is designed to detect tobramycin (TOB) drug applying a hybrid structure of three aptamer strands and SYBR Green I (SGI) fluorescent dye as the bioreceptor segment and signal indicator, respectively. The preferential binding of the aptamers to TOB resulted in the collapse of the hybridized aptamer skeleton to the single strands. So, the intercalation of SGI molecules reduced that quenched the fluorescence response. The aptasensing assay provided the superior target specificity with a detection limit (LOD) of 0.153 pM and a wide linear dynamic range over 0.5 pM-300 μM. The aptasensor could successfully quantify TOB in human serum samples. The tag-free sensor with the remarkable advantages of simplicity, easy-to-use, cost-effectiveness, and high sensitivity is superior to be applicable for clinical samples.
科研通智能强力驱动
Strongly Powered by AbleSci AI