化学
生物分子
荧光
水热碳化
细菌
检出限
金黄色葡萄球菌
碳化
纳米技术
色谱法
生物化学
有机化学
材料科学
量子力学
生物
物理
吸附
遗传学
作者
Chen Deng,Hui Li,S.‐B. Qian,Pan Fu,Hualan Zhou,Zheng Jian,Yuhui Wang
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2022-08-12
卷期号:94 (33): 11514-11520
被引量:13
标识
DOI:10.1021/acs.analchem.2c01430
摘要
By virtue of the fascinating merits of low cost, rapid screening, and on-site detection, fluorescence lateral flow assays (FLFAs) have attracted considerable attention. Their detection limits are closely associated with the label probes used. The development of high-performance and robust phosphors remains a great challenge. Herein, we presented a new label probe, i.e., fluorescent carbon nanobeads (FCNBs), for FLFAs. Monodispersive, water-soluble, and highly emissive FCNBs were facilely prepared via a hydrothermal carbonization manner. Their abundant amino groups were beneficial for versatile surface functionalization. After being modified by biomolecules, the fabricated FCNB reporter probes were adopted for the construction of lateral flow test strips toward representative foodborne toxins, i.e., aflatoxin B1 (AFB1), and pathogenic bacteria, i.e., Staphylococcus aureus (S. aureus), respectively. The detection limits (0.01 ng/mL for AFB1 and 102 cfu/mL for S. aureus) were about 1 or 2 orders of magnitude lower than most reported methods. Furthermore, the proposed test strips were successfully applied for the quantitative, accurate, and rapid screening of AFB1 and S. aureus in food samples. This work provided a promising label probe for FLFAs and would open the opportunity to exploit a sensing platform by modifying different ligands onto the FCNBs.
科研通智能强力驱动
Strongly Powered by AbleSci AI