已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Polydopamine/IR820 nanoparticles as topical phototheranostics for inhibiting psoriasiform lesions through dual photothermal and photodynamic treatments

光动力疗法 化学 角质形成细胞 光热治疗 哈卡特 细胞毒性 生物物理学 光毒性 体内 伊米奎莫德 活性氧 体外 纳米技术 材料科学 免疫学 生物化学 医学 生物 生物技术 有机化学
作者
G.R. Nirmal,Zih‐Chan Lin,Chih-Hung Lin,Calvin T. Sung,Chia-Chih Liao,Jia‐You Fang
出处
期刊:Biomaterials Science [The Royal Society of Chemistry]
卷期号:10 (21): 6172-6189 被引量:13
标识
DOI:10.1039/d2bm00835a
摘要

Dual photothermal and photodynamic therapy (PTT and PDT) is an attractive approach that generates a synergistic effect for inhibiting keratinocyte hyperproliferation in the treatment of psoriasis. Here, we developed phototheranostic nanocarriers capable of producing hyperthermia and reactive oxygen species (ROS) in response to near-infrared (NIR) illumination. To this end, IR820 with photothermal and photodynamic features was embedded in nano-sized polydopamine (PDA) acting as a PTT agent. A comprehensive characterization of the PDA/IR820 nanosystem was performed according to its morphology, size, zeta potential, UV absorbance, and heat generation. Its therapeutic efficacy was assessed by a keratinocyte-based study and using an imiquimod (IMQ)-stimulated psoriasiform murine model. PDA/IR820 nanoparticles were facilely internalized into keratinocytes and mainly resided in lysosomes. Upon irradiation with NIR light, ROS were generated inside the keratinocytes to cause a photodynamic effect. The live/dead cell assay and cytotoxicity assay demonstrated that PDA and IR820 acted as effective photoabsorbers to induce keratinocyte death. The highest cytotoxic effect was detected in the group of NIR-irradiated PDA/IR820 nanoparticles, which killed 52% of keratinocytes. The nanosystem acted through the caspase and poly ADP-ribose polymerase (PARP) pathways to induce keratinocyte apoptosis. In vitro and in vivo skin permeation indicated the selective accumulation of the topically applied PDA/IR820 nanoparticles within psoriasiform skin, suggesting their skin-targeting capability. The combination of PDA/IR820 nanoparticles and NIR irradiation increased the skin temperature by 11.7 °C. PTT/PDT eliminated psoriasiform plaques in mice by decreasing hyperplasia, inhibiting cytokine overexpression, and recovering the barrier function. The epidermal thickness of the IMQ-treated skin was reduced from 134 to 34 μm by the nanocarriers plus NIR. The IR820 nanoparticles were largely deposited on the inflamed areas of psoriasiform lesions for monitoring the severity of inflammation. The image-guided phototheranostic nanoparticles showed their potential for applications in psoriasis management via noninvasive topical administration.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
义气珩完成签到,获得积分10
2秒前
2秒前
Banff完成签到 ,获得积分10
3秒前
3秒前
3秒前
英姑应助天润佳苑采纳,获得10
5秒前
幼儿园老大完成签到,获得积分10
6秒前
聪明安筠发布了新的文献求助10
7秒前
Ash发布了新的文献求助10
8秒前
狂野诗柳关注了科研通微信公众号
9秒前
smy发布了新的文献求助10
10秒前
xvzhenyuan完成签到,获得积分10
10秒前
11秒前
13秒前
聪明安筠完成签到,获得积分10
13秒前
14秒前
Jasper应助雅香采纳,获得10
15秒前
耶?发布了新的文献求助10
15秒前
桓某人发布了新的文献求助10
18秒前
18秒前
王琳琳完成签到 ,获得积分10
19秒前
20秒前
万能图书馆应助标致的愫采纳,获得10
20秒前
20秒前
啦啦啦啦发布了新的文献求助10
22秒前
22秒前
双黄应助赵小楠采纳,获得50
23秒前
zhouxu发布了新的文献求助10
23秒前
23秒前
打打应助smy采纳,获得10
25秒前
小马甲应助陈星锦采纳,获得10
25秒前
天道酬勤完成签到,获得积分10
27秒前
27秒前
科研通AI2S应助Ash采纳,获得10
28秒前
双黄应助王九八采纳,获得10
29秒前
30秒前
ding应助茶蛋采纳,获得10
30秒前
朗源Wu发布了新的文献求助10
37秒前
双黄应助赵小楠采纳,获得50
42秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248513
求助须知:如何正确求助?哪些是违规求助? 2891915
关于积分的说明 8269223
捐赠科研通 2559929
什么是DOI,文献DOI怎么找? 1388807
科研通“疑难数据库(出版商)”最低求助积分说明 650897
邀请新用户注册赠送积分活动 627798