甲状旁腺激素
医学
维生素D与神经学
内科学
置信区间
内分泌学
方差分析
铁状态
免疫分析
钙
免疫学
抗体
缺铁
贫血
作者
Francesca Iannone,Elvira Angotti,Fortunata Lucia,Luisa De Martino,Giulio Cesare Antico,Francesco Galato,Ilenia Aversa,Raffaella Gallo,Caterina Giordano,Antonio Abatino,S Mancuso,Lorenza Giaquinto Carinci,Maria Martucci,Consuelo Teti,Francesco Costanzo,Giovanni Cuda,Camillo Palmieri
标识
DOI:10.1016/j.cca.2024.117863
摘要
Measuring 1,25-dihydroxyvitamin D (1,25(OH)2D), parathyroid hormone 1–84 (PTH 1–84) and intact FGF23 (iFGF23) is crucial for diagnosing a variety of diseases affecting bone and mineral homeostasis. Biological variability (BV) data are important for defining analytical quality specifications (APS), the usefulness of reference intervals, and the significance of variations in serial measurements in the same subject. The aim of this study was to pioneer the provision of BV estimates for 1,25(OH)2D and to improve existing BV estimates for iFGF23 and PTH 1–84. Serum and plasma-EDTA samples of sixteen healthy subjects have been collected for seven weeks and measured in duplicate by chemiluminescent immunoassay on the DiaSorin Liaison platform. After variance verification, within-subject (CVI) and between-subject (CVG) BV estimates were assessed by either standard ANOVA, or CV-ANOVA. The APSs were calculated according to the EFLM-BV-model. We found the following CVI estimates with 95% confidence intervals:1,25(OH)2D, 22.2% (18.9–26.4); iFGF23, 16.1% (13.5–19.5); and PTH 1–84, 17.9% (14.8–21.8). The CVG were: 1,25(OH)2D, 21.2% (14.2–35.1); iFGF23, 21.1% (14.5–35.8); and PTH 1–84, 31.1% (22.1–50.8). We report for the first time BV estimates for 1,25(OH)2D and enhance existing data about iFGF23-BV and PTH 1–84-BV through cutting-edge immunometric methods.
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