亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Serum exosomal microRNA pathway activation in placenta accreta spectrum: pathophysiology and detection

小RNA 微泡 胎盘 外体 医学 胎盘植入 免疫印迹 生物标志物 男科 病理 生物 生物信息学 怀孕 胎儿 基因 遗传学
作者
Jessian L. Munoz,Brett D. Einerson,Robert M. Silver,Sureshkumar Mulampurath,Lauren S. Sherman,Pranela Rameshwar,Egle Bytautiene,Patrick S. Ramsey
出处
期刊:AJOG global reports [Elsevier]
卷期号:4 (1): 100319-100319 被引量:2
标识
DOI:10.1016/j.xagr.2024.100319
摘要

Placenta Accreta Spectrum (PAS) disorders are a complex range of placental pathologies associated with significant maternal morbidity and mortality. Diagnosis of PAS relies on ultrasonographic findings with modest positive predictive value. Exosomal microRNA (miR) are small RNA molecules which reflect the cellular processes of origin tissues. We aimed to explore exosomal miR expression to understand PAS pathology and clinical utility for PAS detection. This study was a biomarker analysis of prospectively collected samples in two academic institutions from 2011-2022. Plasma specimens were collected from patients with suspected PAS, placenta previa or repeat cesarean section. Exosomes were quantified and characterized by nanoparticle tracking analysis and Western blot. MiR were assessed by PCR array and targeted single quantification. MiR pathway analysis was performed using Ingenuity® Pathway Analyses software. Placental biopsies were taken from all groups and analyzed by PCR and whole cell ELISA. ROC univariate analysis was performed for microRNA prediction of PAS. Clinically relevant outcomes were collected from abstracted medical records. Plasma specimens were analyzed from a total of 120 subjects (60 PAS, 30 placenta previa and 30 control). Isolated plasma exosomes had a mean size of 71.5 nm and were ten times greater in PAS specimens (20 vs 2 particles/frame). Protein expression of exosomes were positive for ICAM-1, Flotilin, Annexin and CD9. MiR analysis showed increased detection of three miRs (mir-92/103/192) in patients with PAS. Pathway interaction assessment revealed differential regulation of p53 signaling in PAS and ERBB2/HER2 in control specimens. These findings were subsequently confirmed in placental protein analysis. Placental miR paralleled plasma exosomal miR expression. Biomarker assessment of PAS-signature miR had an AUC=0.81 (p< 0.001, 95% CI [0.73-0.89]) with a sensitivity and specificity of 89.2% and 80%, respectively. In this large cohort, plasma exosomal miR assessment revealed differentially expressed pathways in PAS and are potential biomarkers for the detection of PAS.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
顾矜应助lf采纳,获得10
2秒前
化羽归尘发布了新的文献求助10
5秒前
mwang完成签到,获得积分10
12秒前
13秒前
李健的小迷弟应助Wakakak采纳,获得10
17秒前
lf发布了新的文献求助10
17秒前
Eunice完成签到,获得积分10
18秒前
23秒前
威武的雨筠完成签到 ,获得积分10
29秒前
研友_VZG7GZ应助哈哈哈哈采纳,获得10
32秒前
momochichu发布了新的文献求助10
33秒前
35秒前
35秒前
39秒前
Wakakak发布了新的文献求助10
40秒前
高贵逍遥完成签到 ,获得积分10
40秒前
wyz完成签到 ,获得积分10
41秒前
43秒前
冰激凌完成签到,获得积分10
44秒前
44秒前
Yu发布了新的文献求助10
46秒前
小乘号子发布了新的文献求助30
46秒前
。。完成签到,获得积分10
47秒前
量子星尘发布了新的文献求助10
49秒前
哈哈哈哈发布了新的文献求助10
50秒前
故意的山河完成签到,获得积分10
52秒前
Linda发布了新的文献求助10
54秒前
struggling2026完成签到 ,获得积分10
59秒前
科研通AI5应助Wakakak采纳,获得10
1分钟前
喂喂发布了新的文献求助20
1分钟前
lena完成签到 ,获得积分10
1分钟前
情怀应助小乘号子采纳,获得10
1分钟前
1分钟前
深情安青应助深情访文采纳,获得10
1分钟前
星辰大海应助科研通管家采纳,获得10
1分钟前
wanci应助科研通管家采纳,获得10
1分钟前
共享精神应助科研通管家采纳,获得10
1分钟前
大模型应助科研通管家采纳,获得30
1分钟前
1分钟前
半城微凉应助科研通管家采纳,获得10
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 700
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3976600
求助须知:如何正确求助?哪些是违规求助? 3520700
关于积分的说明 11204482
捐赠科研通 3257320
什么是DOI,文献DOI怎么找? 1798683
邀请新用户注册赠送积分活动 877881
科研通“疑难数据库(出版商)”最低求助积分说明 806613