羧甲基纤维素
自愈水凝胶
组织工程
聚乙烯醇
细胞包封
海藻酸钙
化学
生物物理学
聚合物
再生(生物学)
化学工程
控制释放
材料科学
钙
生物医学工程
高分子化学
钠
纳米技术
细胞生物学
有机化学
工程类
生物
医学
作者
Atefeh Akbari,Rahman Emamzadeh,Mahboobeh Nazari,Thomas Brandstëtter,Jürgen Rühe
标识
DOI:10.1016/j.ijbiomac.2023.128846
摘要
The growth factor Anterior Gradient 2 (AGR2) has been shown to have an effective role in tissue regeneration, but remained largely unexplored in localized tissue engineering applications. Alginate beads have been proven as safe carriers for protein encapsulation, but they suffer from fragility and uncontrolled protein release. For such alginate systems, little is known about how changes in concentrations and ion-crosslinking affect protein release and accumulation in 3-D matrices. To address these questions, an engineered interpenetrating polymer network (IPN) has been used to synthesize a novel hybrid system consisting of AGR2 loaded beads composed of calcium-crosslinked sodium alginate (SA) and carboxymethyl cellulose (CMC). These beads are embedded in films consisting of SA and polyvinyl alcohol (PVA), using a simple ion gelation technique. We assess protein release kinetics and accumulation within the hybrid system by varying polymer concentrations and cross-linking parameters. The IPN hybrid system maintains controlled release over two weeks, without an initial burst period. Through this approach efficicnt delivery of AGR2 is achieved which in turn effectively mediates cell migration and proliferation, resulting in excellent cell viability and complete wound closure. The described release system opens new perspectives in tissue engineering.
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