没食子酸
白色念珠菌
白色体
微生物学
效力
最小抑制浓度
肉汤微量稀释
生物化学
化学
IC50型
生物
体外
抗菌剂
抗氧化剂
作者
Velasco Valderrama,P. Sanchéz-Sáez,Macarena Delso,Mario Díaz‐Dosque,Alejandro Escobar,Mauricio Budini,Mabel Catalán,Raúl Vivar,Rodrigo López‐Muñoz,José A. Jara,Alfredo Molina‐Berríos
标识
DOI:10.1093/jambio/lxad316
摘要
Abstract Aims To evaluate the antifungal and antibiofilm activity of gallic acid derivatives TPP+-C10 and TPP+-C12 and their effects on mitochondrial function on two Candida albicans reference strains (ATCC 90029 and ATCC 10231). Methods and results First, we determined minimal inhibitory concentration (MIC) using a microdilution assay. Both compounds exerted antifungal effects, and their MICs ranged from 3.9 to 13 µM, with no statistically significant differences between them (P > 0.05, t-test). These concentrations served as references for following assays. Subsequently, we measured oxygen consumption with a Clark electrode. Our observations revealed that both drugs inhibited oxygen consumption in both strains with TPP+-C12 exerting a more pronounced inhibitory effect. We then employed flow cytometry with TMRE as a probe to assess mitochondrial membrane potential. For each strain assayed, the compounds induced a decay in transmembrane potential by 75%–90% compared to the control condition (P < 0.05, ANOVA). Then, we measured ATP levels using a commercial kit. TPP+-C12 showed a 50% decrease of ATP content (P < 0.05 ANOVA), while TPP+-C10 exhibited a less pronounced effect. Finally, we assessed the antibiofilm effect using the MTT reduction assay. Both compounds were effective, but TPP+-C12 displayed a greater potency, requiring a lower concentration to inhibit 50% of biofilms viability (P < 0.05, t-test). Conclusions Derivatives of gallic acid linked to a TPP+ group exert antifungal and antibiofilm activity through impairment of mitochondrial function in C. albicans.
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