A piezoelectric micromachined ultrasound transducer combined with recording electrodes for acute brain preparations in vitro

神经调节 超声波 生物医学工程 局部场电位 超声波传感器 传感器 切片制备 脑-机接口 刺激 材料科学 微电极 神经科学 电生理学 医学 化学 声学 电极 脑电图 生物 放射科 物理 物理化学
作者
Ryo Furukawa,Takahiro Yoshikawa,Shuichi MURAKAMI,Takashi Tateno
出处
期刊:Journal of Neuroscience Methods [Elsevier BV]
卷期号:403: 110048-110048
标识
DOI:10.1016/j.jneumeth.2023.110048
摘要

Ultrasound stimulation is used to noninvasively stimulate the local and deep areas of the brain. However, the detailed cellular mechanisms of neural activation are still unclear because studies on micro-stimulation at the cellular level are lacking. To modulate neural activity at the cellular level, we developed a piezoelectric micromachined ultrasound transducer (PMUT), having circular diaphragms for application on acute brain slice preparations. To monitor neural activities, additionally, we fabricated recording microelectrodes onto the same PMUT device for closed-loop application. To examine the PMUT-driven cellular responses of a brain slice, intracellular calcium signals in individual cells were measured using two calcium indicators. We successfully observed the intracellular responses triggered by the ultrasound of our novel PMUT. In addition, we performed recordings of local field potentials in a brain slice, demonstrating its usefulness as a simultaneous recording interface. Conventional ultrasound stimulators are open-loop systems that risk inducing excessive neural activity because of the absence of neural activity monitoring. In contrast, our PMUT is packaged in a single device with both stimulation and sensor interface for neuromodulation. Further, there are no published reports on in vitro microdevices that can be used for ultrasound stimulation in rodent cortical slices that are several hundred micrometers thick, which maintain the cortical laminar structure and intrinsic neural networks. Our findings suggest that this novel PMUT device has the potential for being a powerful tool for in vitro brain slice applications and effective closed loop ultrasound stimulation.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
yar应助体贴凌柏采纳,获得10
1秒前
自由的雪一完成签到,获得积分10
1秒前
Ava应助李振博采纳,获得200
1秒前
JW发布了新的文献求助10
2秒前
无限的千凝完成签到 ,获得积分10
3秒前
CipherSage应助YeeYee采纳,获得10
3秒前
3秒前
Ander完成签到 ,获得积分10
4秒前
化白完成签到,获得积分10
5秒前
H.完成签到,获得积分10
5秒前
chuzihang完成签到 ,获得积分10
5秒前
科研小狗完成签到,获得积分10
11秒前
11完成签到,获得积分10
12秒前
柏林寒冬应助QAQ采纳,获得10
14秒前
Flynn完成签到 ,获得积分10
16秒前
17秒前
17秒前
BZPL完成签到,获得积分10
18秒前
LANER完成签到 ,获得积分10
18秒前
18秒前
ww完成签到 ,获得积分10
18秒前
拉布拉多多不多完成签到,获得积分10
19秒前
多肉丸子完成签到,获得积分10
20秒前
yian完成签到,获得积分10
20秒前
HJJHJH发布了新的文献求助30
21秒前
正直无极完成签到,获得积分10
21秒前
认真的一刀完成签到,获得积分10
21秒前
杨天天完成签到,获得积分0
21秒前
yukiseto发布了新的文献求助30
22秒前
小熊爱吃糖完成签到,获得积分10
23秒前
李振博发布了新的文献求助200
24秒前
realityjunky完成签到,获得积分10
24秒前
peekaboo完成签到,获得积分10
24秒前
高贵的迎蕾完成签到 ,获得积分10
26秒前
wqc2060完成签到,获得积分10
26秒前
曾经小伙完成签到 ,获得积分10
26秒前
赘婿应助realityjunky采纳,获得10
29秒前
温柔的蛋挞完成签到,获得积分10
29秒前
whuhustwit完成签到,获得积分10
30秒前
30秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038303
求助须知:如何正确求助?哪些是违规求助? 3576013
关于积分的说明 11374210
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029