Identification of Multifunctionality of Grass Carp ( <i>Ctenopharyngodon Idella</i>) Beclin1 During Bacterial Infection

Beclin1 is one of the first genes to be proven to positively regulate autophagy. It also connects immunization with autophagy in mammals. In this study, we reported that grass carp Beclin1 gene expression could be upregulated by bacterial infection. Overexpression of Beclin1 could increase the autophagy level of CIK cells by raising the ratio of LC II/I, and decrease the number of adhesive bacteria. Beclin1 could promote cellular migration, proliferation abilities, and vitality, but not anti-apoptosis. Besides, the expression of Beclin1 could activate the NF-κB signaling pathway by strengthening inflammatory cytokines Our finding indicated that Beclin1 participated in Ctenopharyngodon idella innate immune progress and autophagy. This study provides evidence of the positive regulation of Beclin1 in teleost innate immunity with its multiple functions. It thus may provide important information on the defenses and immune mechanisms of teleoskeletal fish against pathogens.