免疫学
免疫
嗜酸性粒细胞增多症
支气管肺泡灌洗
免疫系统
粘液
医学
获得性免疫系统
生物
肺
内科学
生态学
作者
Kunyuan Tian,Prakrati Dangarh,Haina Zhang,Cory Hines,Andrew Bush,Hannah J. Pybus,James A. Harker,Clare M. Lloyd,Reiko Tanaka,Sejal Saglani
摘要
Abstract Background Preschool wheeze attacks triggered by recurrent viral infections, including respiratory syncytial virus (RSV), are associated with an increased risk of childhood asthma. However, mechanisms that lead to asthma following early‐life viral wheezing remain uncertain. Methods To investigate a causal relationship between early‐life RSV infections and onset of type 2 immunity, we developed a neonatal murine model of recurrent RSV infection, in vivo and in silico, and evaluated the dynamical changes of altered airway barrier function and downstream immune responses, including eosinophilia, mucus secretion and type 2 immunity. Results RSV infection of neonatal BALB/c mice at 5 and 15 days of age induced robust airway eosinophilia, increased pulmonary CD4 + IL‐13 + and CD4 + IL‐5 + cells, elevated levels of IL‐13 and IL‐5 and increased airway mucus at 20 days of age. Increased bronchoalveolar lavage albumin levels, suggesting epithelial barrier damage, were present and persisted following the second RSV infection. Computational in silico simulations demonstrated that recurrent RSV infection resulted in severe damage of the airway barrier (epithelium), triggering the onset of type 2 immunity. The in silico results also demonstrated that recurrent infection is not always necessary for the development of type 2 immunity, which could also be triggered with single infection of high viral load or when the epithelial barrier repair is compromised. Conclusions The neonatal murine model demonstrated that recurrent RSV infection in early life alters airway barrier function and promotes type 2 immunity. A causal relationship between airway barrier function and type 2 immunity was suggested using in silico model simulations.
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