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Identification of Isthmin‐1 in common carp (Cyprinus carpio L.) and the effects on glucose metabolism in vivo and in vitro

生物 鲤鱼 肝胰腺 挑剔 碳水化合物代谢 鲤鱼 体内 基因表达 生物化学 内分泌学 内科学 基因 遗传学 渔业 医学
作者
Leya Qu,Liping Yang,Yiran Wang,Shaoyang Zhi,Mengjuan Zhao,Jinrui Xiong,Xiao Yan,Chaobin Qin,Guoxing Nie
出处
期刊:Journal of Fish Biology [Wiley]
卷期号:104 (5): 1483-1492
标识
DOI:10.1111/jfb.15681
摘要

Abstract Isthmin‐1 (Ism1) plays roles in glucose uptake in mammals as an adipokine. To investigate its role in the glucose metabolism of common carp ( Cyprinus carpio. L), the Ism1 sequence was cloned, and its expression and distribution in tissues were detected. In addition, we prepared and purified the recombinant Ism1 protein using the E. coli expression system and assessed changes in the expression of key genes related to glucose metabolism through both in vivo injection experiments and primary hepatocyte experiments in vitro. The results revealed that the open reading frame of Ism1 was 1377 bp long, encoding 458 amino acids. Similarity analysis indicated that Ism1 exhibited a close evolutionary relationship with goldfish ( Carassius auratus ), sharing 98.35% amino acid similarity. Ism1 was expressed in all tissues of common carp, with the highest level observed in the heart, followed by the gill, head kidney, and hepatopancreas. Distinct patterns of Ism1 expression were identified during the oral glucose tolerance test and long‐term high‐carbohydrate and high‐fat diet feeding experiments. In vivo studies demonstrated that the serum glucose concentration was reduced on treatment with Ism1, accompanied by a significant upregulation of mRNA levels for gk , hk , and pfk genes in hepatopancreas; conversely pepck and g6pase mRNA levels were significantly downregulated in the hepatopancreas under these conditions as well. Furthermore, our primary hepatocyte experiment confirmed that Ism1 could inhibit pepck and g6pase mRNA expression, while promoting gk , hk , and pfk mRNA expression levels. In conclusion, Ism1, in common carp, could participate in the glucose metabolism, which provides essential information for future studies on the function of Ism1.
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