Baicalin suppresses macrophage JNK-mediated adipose tissue inflammation to mitigate insulin resistance in obesity

胰岛素抵抗 炎症 脂肪组织 巨噬细胞 黄芩苷 肥胖 医学 脂肪组织巨噬细胞 胰岛素 内分泌学 内科学 生物 化学 生物化学 体外 高效液相色谱法 色谱法
作者
Ziyue Zhu,Mei Yu,Mengfan Xu,Xin Ji,Xicui Zong,Zhenwen Zhang,Wenbin Shang,Li Zhang,Penghua Fang
出处
期刊:Journal of Ethnopharmacology [Elsevier]
卷期号:332: 118355-118355 被引量:1
标识
DOI:10.1016/j.jep.2024.118355
摘要

Radix scutellariae (the root of Scutellaria baicalensis Georgi) is a traditional Chinese medicine (TCM) used to treat a wide range of inflammation-related diseases, such as obesity, diabetes, diabetic kidney disease, and COVID-19-associated inflammatory states in the lung and kidney. Baicalin is the major anti-inflammatory component of Radix scutellariae and has shown the potential to inhibit inflammation in metabolic disorders. In this study, we explored the ability and underlying mechanisms of baicalin to modulate the macrophage to mitigate insulin resistance in obesity. Obese mice were administered baicalin (50 mg/kg/day) intraperitoneally for 3 weeks. RAW264.7 and BMDM cells were stimulated with LPS and treated with baicalin for 24 h, while 3T3-L1 and primary white adipocytes were treated with the supernatants from baicalin-treated RAW264.7 cells for 24 h. The results showed that baicalin significantly improved glucose and insulin tolerance as well as decreased fat and adipose tissue macrophage levels in obese mice. Besides, baicalin significantly reduced serum and adipose tissue IL-1β, TNF-α and IL-6 levels in obese mice, as well as suppressed LPS-induced IL-1β, TNF-α and IL-6 expression and release in macrophages. Furthermore, treatment with the supernatant from baicalin-treated RAW264.7 cells increased the levels of PGC-1α, SIRT1, p-IRS-1 and p-AKT in adipocytes. Moreover, baicalin treatment dramatically downregulated macrophage p-p38, p-JNK, and Ac-p65Lys310 levels while increasing SIRT1 both in vivo and in vitro. Importantly, JNK inhibitor SP600125 blocked most of the effects of baicalin on SIRT1, Ac-p65Lys310 and pro-inflammatory factors in macrophages. Therefore, these results demonstrated for the first time that baicalin exerts its anti-inflammatory effects in obese adipose tissue macrophages mainly through suppressing JNK/SIRT1/p65 signaling. These findings amplified the mechanisms of baicalin and its potential to attenuate insulin resistance.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
apple9515完成签到 ,获得积分10
1秒前
2秒前
Amadeus发布了新的文献求助30
2秒前
rot完成签到 ,获得积分10
2秒前
6秒前
7秒前
神勇的天问完成签到 ,获得积分10
9秒前
研友_VZG7GZ应助wang采纳,获得10
9秒前
CipherSage应助科研通管家采纳,获得10
12秒前
12秒前
Ava应助科研通管家采纳,获得10
12秒前
Singularity应助科研通管家采纳,获得20
12秒前
12秒前
Re_move完成签到,获得积分10
12秒前
quhayley应助科研通管家采纳,获得20
12秒前
Singularity应助科研通管家采纳,获得10
12秒前
小马甲应助科研通管家采纳,获得10
12秒前
12秒前
12秒前
沐风发布了新的文献求助10
16秒前
腼腆的人生完成签到,获得积分10
17秒前
今后应助Amadeus采纳,获得10
18秒前
越红完成签到,获得积分10
20秒前
wang完成签到,获得积分10
20秒前
20秒前
21秒前
24秒前
顾矜应助爱杨紫的土豆子采纳,获得10
26秒前
安全网123完成签到,获得积分10
28秒前
Jing完成签到 ,获得积分10
29秒前
李爱国应助CDH采纳,获得10
30秒前
30秒前
江江江江江江江江完成签到,获得积分20
30秒前
31秒前
风趣青槐完成签到,获得积分10
32秒前
pacify完成签到 ,获得积分10
33秒前
找不到完成签到,获得积分0
33秒前
小夜完成签到,获得积分10
34秒前
35秒前
KDC发布了新的文献求助10
36秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Genera Insectorum: Mantodea, Fam. Mantidæ, Subfam. Hymenopodinæ (Classic Reprint) 800
Ethnicities: Media, Health, and Coping 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3085875
求助须知:如何正确求助?哪些是违规求助? 2738737
关于积分的说明 7551604
捐赠科研通 2388581
什么是DOI,文献DOI怎么找? 1266613
科研通“疑难数据库(出版商)”最低求助积分说明 613527
版权声明 598591