Involvement of glyphosate in disruption of biotransformation P450 enzymes and hepatic lipid metabolism in chicken

AbstractAbstractThe current study investigated the potentially harmful consequences of pure glyphosate or Roundup® on CYP family members and lipid metabolism in newly hatched chicks. On the sixth day, 225 fertilized eggs were randomly divided into three treatments: (1) the control group injected with deionized water, (2) the glyphosate group injected with 10 mg pure glyphosate/Kg egg mass and (3) the Roundup group injected 10 mg the active ingredient glyphosate in Roundup®/Kg egg. The results of the study revealed a reduction in hatchability in chicks treated with Roundup®. Moreover, change of Lipid concentration in serum and the liver-treated groups. Additionally, increased liver function enzymes and increased oxidative stress in the glyphosate and Roundup® groups. Furthermore, liver tissues showed histological changes and several lipid deposits in glyphosate-treated groups. Hepatic CYP1A2 and CYP1A4 expressions were significantly increased (p < .05) after glyphosate exposure, and suppression of CYP1C1 mRNA expression was significant (p < .05) after Roundup® exposure. The pro-inflammatory cytokines genes IFN-γ and IL-1β expression were significantly increased (p < .05) after Roundup® exposure. In addition, there were significant differences in the levels of expression genes which are related to lipid synthesis or catabolism in the liver. In conclusion, in ovo glyphosate exposure caused disruption of biotransformation, pro-inflammatory and lipid metabolism in chicks.Keywords: GlyphosateRoundup®chickshepatic lipid metabolismin ovo Disclosure statementNo potential conflict of interest was reported by the author(s).Authors contributionsAll authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by [Mohamed Ahmed Fathi], [Dan Shen], [Adel Mohamed Abdelsalam and [Chunmei Li]. The first draft of the manuscript was written by [Mohamed Ahmed Fathi], and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.Additional informationFundingThis study was supported by the earmarked fund for Jiangsu Agricultural Industry Technology System (JATS [2022]480) and the National Nature Science Foundation of China (No. 32072781).