脱氧核酶
噬菌体展示
化学
小RNA
寡核苷酸
计算生物学
滚动圆复制
检出限
酶
生物
分子生物学
DNA
生物化学
基因
肽
聚合酶
色谱法
作者
Yan Zeng,Yue Hui,Binrui Cao,Yan Li,Mingying Yang,Chuanbin Mao
标识
DOI:10.1002/anie.202210121
摘要
Integrating artificial enzymes onto nanostructures target- and site-specifically is still a challenge. Here we show that target miRNAs trigger the formation of DNAzyme site-specifically at the tip of filamentous phage for detecting miRNA biomarkers. Through an antibody-modified oligonucleotide, the tip of the phage with magnetic nanoparticles on the sidewall captures a target miRNA, inducing the formation of DNAzyme that extends from the phage tip through a hybridization chain reaction. After magnetic separation, the resultant complex catalyzes a colorimetric reaction with the signal correlated to target concentrations, leading to the quantification of miRNAs with a detection limit of 5.0 fM, about 103 folds lower than the phage-free approach. Our approach can differentiate miRNA mutants and quantify miRNA in human plasma, tumor cells, and tissues with high sensitivity, suggesting that the target-triggered integration of enzymes and phages holds promise for searching for new catalysts.
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