链球菌
肠道菌群
肠球菌
细菌
粪便
短链脂肪酸
微生物学
放线菌门
脂肪酸
生物
微生物群
厚壁菌
食品科学
生理学
16S核糖体RNA
抗生素
生物化学
生物信息学
发酵
丁酸盐
遗传学
作者
M. Duan,Z.H. Han,Taotao Huang,Yuzhuo Yang,Bao Huang
摘要
This study aims to investigate the gut microbiota and metabolites in breastfed infants with breast milk jaundice (BMJ) using gut microbiome–metabolomics. Breastfed newborns diagnosed with BMJ and those without BMJ (control group) were enrolled. Faecal samples were collected from the participants and subjected to high‐throughput sequencing of the 16s rDNA V3 and V4 regions of the gut flora and metabolomics of short‐chain fatty acids (SCFAs). Proteobacteria, Fimicutes and Actinobacteria were the main bacteria at the phylum level. Eshcerichia‐Shigella and Enterobacteriacea were the main bacteria at the genus level. The difference between the two groups was compared. Compared to the control group, the amount of Streptococcus was significantly increased while the amount of Enterococcus was significantly decreased in the faeces from infants with BMJ. Functional prediction analysis of 16S found that biosynthesis of penicillin and cephalosporin significantly increased in the BMJ group. Gas chromatography–mass spectrometry detection of SCFAs revealed that levels of acetic acid and propionic acid were significantly lower in the BMJ group than in the control group. The reduced levels of acetic acid and propionic acid may be related to the increase in Streptococcus and decrease in Enterococcus, both of which may contribute to BMJ.
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