类有机物
印记(心理学)
α-突触核蛋白
肽
共核细胞病
帕金森病
神经突
分子印迹
化学
生物物理学
神经科学
生物
医学
病理
生物化学
体外
选择性
催化作用
疾病
基因
作者
Mei-Hwa Lee,James L. Thomas,Zi-Lin Su,Wen–Kuan Yeh,Anna S. Monzel,Silvia Bolognin,Jens C. Schwamborn,Chien‐Hsin Yang,Hung‐Yin Lin
标识
DOI:10.1016/j.bios.2020.112852
摘要
Parkinson's disease (PD) is a progressive nervous system disorder that affects movement, whose early signs may be mild and unnoticed. α-Synuclein has been identified as the major component of Lewy bodies and Lewy neurites, which are the characteristic proteinaceous deposits that are the hallmarks of PD. In this work, three alpha-synuclein peptides were synthesized as templates for the molecular imprinting of conductive polymers to enable recognition of alpha-synuclein via ultrasensitive electrochemical measurements. The peptide sequences encompassed specific residues where mutations are known to accelerate PD (though the target sequences, in this study, were wild-type.) The different peptide targets were all successfully imprinted, but with differing imprinting effectiveness, probably owing to differences in target carboxylic acids (which can bind to the aniline (AN) m-aminobenzenesulfonic acid (MSAN) MIP polymers.) Composition of the imprinted polymer, (the mole proportions of AN and MSAN), and the concentrations and sequences of imprinted peptide templates were optimized by measuring the electrochemical responses to target peptides. The imprinted electrode can detect alpha-synuclein at fg/mL levels, and was therefore used to measure alpha-synuclein in the culture medium of human brain organoids generated from normal and idiopathic PD patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI