Preparation and Ultrasonic Imaging Investigation of Perfluoropentane-Filled Polylactic Acid Nanobubbles As a Novel Targeted Ultrasound Contrast Agent

聚乳酸 材料科学 超声波 Zeta电位 超声波传感器 分散性 体内 生物医学工程 聚合物 透射电子显微镜 生物物理学 纳米技术 纳米颗粒 高分子化学 复合材料 物理 生物技术 生物 医学 声学
作者
Ruolei Xiao,Zhiwei Zhao,Jiajuan Chen,Liu He,Huili Wang,Lingping Huang,Binhua Luo
出处
期刊:Frontiers in Materials [Frontiers Media]
卷期号:7 被引量:6
标识
DOI:10.3389/fmats.2020.549002
摘要

In the study reported here, polylactic acid (PLLA) polymer was synthesized using stannous octoate (Sn(Oct) 2) and N-(t-butoxycarbonyl) ethanolamine (EABoc) as the catalyst and the initiator, respectively. The selected PLLA polymer with proper molecular weight was used to prepare nanobubbles encapsulating with liquid perfluoropentane. Then, lactoferrin (Lf), which has a good affinity with tumor cells, was conjugated to PLLA nanobubbles. The resulting Lf–PLLA nanobubbles were examined from the perspective of appearance, size, zeta potential, and stability in vitro . The average hydrodynamic diameter of the Lf–PLLA nanobubbles was 315.3 ± 4.2 nm, the polydispersity index (PDI) was 0.153 ± 0.020, and the zeta potential was around −11.3 ± 0.2 mV. Under the transmission electron microscope (TEM), Lf–PLLA nanobubbles were highly dispersed and had a spherical shape with a distinct capsule structure. The Lf–PLLA nanobubbles also showed little cytotoxicity and low hemolysis rate and exhibited good stability in vitro . The enhanced ultrasound imaging ability of Lf–PLLA nanobubbles was detected by an ultrasound imaging system. The results of ultrasound studies in vitro showed that the liquid perfluoropentane underwent phase transition under ultrasonic treatment, which proved the Lf–PLLA nanobubbles could enhance the ability of ultrasonic imaging. The studies of ultrasonic imaging in nude mice bearing subcutaneous tumors showed that the ability of enhanced ultrasonic images was apparent after injection of Lf–PLLA nanobubbles. Acoustic behavior in vitro and in vivo showed that the Lf–PLLA nanobubbles were characterized by strong, stabilized, and the ability of tumor-enhanced ultrasound imaging. Thus, the Lf–PLLA nanobubbles are an effective ultrasound contrast agent for contrast-enhanced imaging.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
QixuGuo完成签到,获得积分10
刚刚
lhy12345完成签到 ,获得积分10
刚刚
naturehome完成签到,获得积分10
1秒前
1秒前
酆雅柔完成签到 ,获得积分10
1秒前
奋斗的珍完成签到,获得积分10
2秒前
海伯利安完成签到,获得积分10
2秒前
RLLLLLLL完成签到 ,获得积分10
2秒前
社牛小柯完成签到,获得积分10
2秒前
vitamin发布了新的文献求助10
3秒前
Fairy完成签到,获得积分10
4秒前
wangzian完成签到 ,获得积分10
4秒前
4秒前
wasfey完成签到,获得积分10
4秒前
4秒前
4秒前
yjzzz完成签到,获得积分10
5秒前
yang完成签到,获得积分10
5秒前
5秒前
晓凡发布了新的文献求助10
6秒前
深情安青应助易琚采纳,获得10
6秒前
galvin完成签到,获得积分10
6秒前
大胆的大有完成签到,获得积分10
6秒前
kuiuLinvk完成签到,获得积分10
6秒前
6秒前
天涯赤子完成签到,获得积分10
7秒前
7秒前
7秒前
7秒前
香蕉书琴发布了新的文献求助10
7秒前
张卷卷完成签到,获得积分10
8秒前
DRHOUSE完成签到,获得积分10
8秒前
无花果应助何困困不困采纳,获得10
8秒前
Ll_l完成签到,获得积分10
9秒前
9秒前
长北关注了科研通微信公众号
9秒前
天天快乐应助虹虹采纳,获得10
10秒前
闪闪的采梦关注了科研通微信公众号
10秒前
10秒前
tengfei发布了新的文献求助10
11秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Social Research Methods (4th Edition) by Maggie Walter (2019) 2390
A new approach to the extrapolation of accelerated life test data 1000
北师大毕业论文 基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 390
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
Robot-supported joining of reinforcement textiles with one-sided sewing heads 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4009325
求助须知:如何正确求助?哪些是违规求助? 3549162
关于积分的说明 11301105
捐赠科研通 3283572
什么是DOI,文献DOI怎么找? 1810370
邀请新用户注册赠送积分活动 886205
科研通“疑难数据库(出版商)”最低求助积分说明 811301