[Overexpression of Klotho inhibits differentiation of RAW264.7 cells into osteoclasts].

Objective To explore the effect of overexpression of Klotho (KL) on the differentiation of RAW264.7 cells intoosteoclasts induced by soluble receptor activator of nuclear factor-κB ligand ( sRANKL). Methods The RAW264.7 cells were divided into Ad-KL group, Ad-GFP group and control group. Inverted microscope was used to observe KL transfected cells. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect mRNA and protein expression level of KL, respectively. Tartrate-resistant acidphosphatase (TRAP) staining was applied to observe the osteoclast differentiation and maturation. The qRT-PCR and Western blotting were performed to assess mRNA or protein expression levels of TRAP and cathepsin K (CTSK). Results Klotho gene was transfected into RAW264.7 cells successfully. Compared with Ad-GFP group and control group, KL mRNA and protein expression levels of Ad-KL group increased significantly. TRAP staining showed that the number and volume of TRAP-positive cells in Ad-KL group were less than those in Ad-GFP group and control group. Compared with Ad-GFP group and control group, TRAP and CTSK mRNA and protein expression levels were reduced remarkably in Ad-KL group. Conclusion Overexpression of Klotho inhibits the differentiation of RAW264.7 cells into osteoclasts.