甲酸
大肠杆菌
甲酸脱氢酶
格式化
化学
拉伤
代谢工程
生物化学
泛醇
氧化酶试验
生物
酶
基因
细胞色素c
线粒体
催化作用
解剖
辅酶Q-细胞色素c还原酶
作者
Bang Junho,Chang Hun Hwang,Jung Ho Ahn,Jong An Lee,Sang Yup Lee
出处
期刊:Nature microbiology
日期:2020-09-28
卷期号:5 (12): 1459-1463
被引量:96
标识
DOI:10.1038/s41564-020-00793-9
摘要
We engineered Escherichia coli to grow on CO2 and formic acid alone by introducing the synthetic CO2 and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo3 and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO2 and formic acid alone. Growth of Escherichia coli on carbon dioxide and formate is achieved by rational metabolic engineering alone.
科研通智能强力驱动
Strongly Powered by AbleSci AI