Naringenin alleviates nonalcoholic steatohepatitis in middle-aged Apoe−/−mice: role of SIRT1

柚皮素 油红O 医学 脂肪变性 脂质过氧化 氧化应激 内科学 化学 药理学 内分泌学 生物 生物化学 类黄酮 体外 抗氧化剂 脂肪生成
作者
Yi Qiao Hua,Yi Zeng,Jin Xu,Xiao Xu
出处
期刊:Phytomedicine [Elsevier]
卷期号:81: 153412-153412 被引量:66
标识
DOI:10.1016/j.phymed.2020.153412
摘要

Naringenin is naturally isolated from citrus fruits possessing many pharmacological activities. However, little is known about the effect of naringenin on nonalcoholic steatohepatitis (NASH) in the model of metabolic syndrome. The present study is aimed to investigate the effect of naringenin on NASH in 12-mo-old male ApoE−/− mice and its possible underlying mechanism. In vivo, 12-mo-old male ApoE−/− mice were administrated with naringenin by intragastric gavage for 12 weeks. At the end of experiment, the blood samples and liver tissues were collected. Metabolic parameters in serum, levels of triglyceride, cholesterol and hydroxyproline, activities of antioxidant enzymes, and content of inflammatory cytokines (TNF-α and IL-6) in liver were examined by corresponding assay kits. Pathological changes in liver were observed by hematoxylin-eosin, oil red O, masson's trichrome, picro-sirius red and senescence β-galactosidase staining. Dihydroethidium was used for detection of reactive oxygen species (ROS). In vitro, AML-12 cells were treated with oleic acid in the presence or absence of naringenin for 24 h. Transfection of SIRT1 siRNA was also conducted in vitro. Lipid accumulation, cellular ROS generation, malondialdehyde content, antioxidant enzyme activities and secretion levels of TNF-α and IL-6 were examined. Both in vivo and in vitro, gene expressions were detected by real-time PCR or western blot. Naringenin administration improved metabolic parameters, suppressed hepatic steatosis, regulated expression of genes involved in lipid metabolism (FASN, SCD1, PPARα and CPT1α), reduced hepatic fibrosis and cell senescence, inhibited hepatic inflammation as evidenced by the decreased macrophage recruitment and content of TNF-α and IL-6, and reduced hepatic oxidative stress by suppressing ROS generation and normalizing activities of antioxidant enzymes. Notably, naringenin administration increased hepatic SIRT1 protein expression and activity along with the increased deacetylation of liver kinase B1 (LKB1), PGC1α and NF-κB. In vitro study, the benefits of naringenin on lipid accumulation, oxidative stress and inflammation were diminished by SIRT1 siRNA transfection. These results indicate that naringenin administration may be a potential curative therapy for NASH treatment and the activation of hepatic SIRT1-mediated signaling cascades is involved in its beneficial effects.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
hua完成签到 ,获得积分10
刚刚
CipherSage应助玉山小霸王采纳,获得10
1秒前
阿飞发布了新的文献求助20
1秒前
吃鲨鱼的小虾米完成签到 ,获得积分20
1秒前
1秒前
我是萨比发布了新的文献求助10
2秒前
科研牛马完成签到,获得积分10
4秒前
Lucas应助小巧日记本采纳,获得10
5秒前
小二郎应助科研通管家采纳,获得10
10秒前
科研通AI2S应助科研通管家采纳,获得30
10秒前
爆米花应助科研通管家采纳,获得10
10秒前
慕青应助科研通管家采纳,获得10
10秒前
上官若男应助科研通管家采纳,获得10
10秒前
李爱国应助科研通管家采纳,获得10
10秒前
我是老大应助科研通管家采纳,获得10
10秒前
爆米花应助科研通管家采纳,获得10
10秒前
Raymond应助科研通管家采纳,获得10
10秒前
quhayley应助科研通管家采纳,获得20
10秒前
淡定剑成应助科研通管家采纳,获得10
10秒前
科研通AI2S应助科研通管家采纳,获得10
10秒前
10秒前
完美世界应助huang采纳,获得10
12秒前
阿飞完成签到,获得积分10
12秒前
14秒前
发两篇Q1完成签到,获得积分20
16秒前
李健的小迷弟应助小米饭采纳,获得10
16秒前
18秒前
19秒前
Bilal完成签到,获得积分10
20秒前
moon完成签到 ,获得积分10
21秒前
21秒前
wuxifan完成签到 ,获得积分10
23秒前
gjww应助chen采纳,获得10
24秒前
CodeCraft应助十一采纳,获得10
24秒前
赘婿应助楚珊珊采纳,获得10
28秒前
29秒前
我是萨比完成签到,获得积分10
30秒前
吉尼太美完成签到,获得积分10
30秒前
30秒前
贪玩的访风完成签到 ,获得积分10
31秒前
高分求助中
The ACS Guide to Scholarly Communication 2500
Sustainability in Tides Chemistry 2000
Pharmacogenomics: Applications to Patient Care, Third Edition 1000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Genera Insectorum: Mantodea, Fam. Mantidæ, Subfam. Hymenopodinæ (Classic Reprint) 800
Ethnicities: Media, Health, and Coping 700
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3085875
求助须知:如何正确求助?哪些是违规求助? 2738737
关于积分的说明 7551604
捐赠科研通 2388581
什么是DOI,文献DOI怎么找? 1266613
科研通“疑难数据库(出版商)”最低求助积分说明 613527
版权声明 598591