斑蝥素
化学
色谱法
体内
碎片(计算)
气相色谱-质谱法
水解
质谱法
代谢物
药理学
生物化学
有机化学
医学
生物
操作系统
生物技术
计算机科学
作者
Chunmin Wei,Yanni Teng,Benjie Wang,Xiumei Zhang,Guiyan Yuan,Xiaoyan Liu,Rong Li,Guo Rui-chen
摘要
Norcantharidin (NCTD), the demethylated analogue of cantharidin, inhibits the proliferation of a variety of human tumor cell lines, and appears to cause the least nephrotoxic and inflammatory side effects. Although NCTD has been used to treat human cancers in China for years, there is no report regarding its metabolism up to now. This is the first report to separate and identify the main metabolites of NCTD in vivo by GC–MS using TMS derivatives. Two hydrolyzed products and five phase I or phase II metabolites were found in rat by the chromatogram comparisons of the blank with incurred biological samples. Multiple stages of fragmentation patterns were used to confirm the metabolites characterizations. The established GC–MS method can also be applied to identifying unknown metabolites of the drugs containing hydroxyl or carbonyl groups in molecular structure.
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