Establishment of green fluorescent protein bone-marrow transplanted mice model and N-methyl nitrosourea-induced prostate cancer mice model

Objective To establish the green fluorescent protein (GFP) bone-marrow transplanted mice model and N-methyl nitrosourea (MNU)-induced model mice model. Methods C57BL/6 mice were irradiated with different dose from 137Cs respectively, then transplanted with new bone-marrow cells, the mouse weight, survival rate and WBC in peripheral blood were obtained to get the optimized 137Cs BMT radiation dose, at a rate of 0.708 Gy/min. Then GFP bone-marrow transplanted mice were established using the optimum dose. The GFP bone-marrow transplanted mice model were tested by flow cytometry analysis. MNU were injected in prostate of C57BL/6 mice combining with testosterone propionate subcutaneous and intraperitoneal injection. The occurrence and progression of prostate cancer were evaluated by hematoxylin and eosin (HE) staining to test the MNU-induced prostate cancer mice model. Results 8.5 Gy was the optimum 137Cs BMT radiation dose, at a rate of 0.708 Gy/min. GFP-positive rate of peripheral non-erythroid cells was (85.3±7.2)% 4 weeks after marrow transplantation, and GFP-positive rate of bone marrow cells was (95.2±2.5)% 28 weeks after marrow transplantation. The tumor incidence of MNU-induced mice was 78% (39/50), and the changing process of the prostate was similar with human in histology. Conclusion The GFP bone-marrow transplanted mice model and MNU-induced model mice model were successfully established. Key words: Bone marrow transplantation; Prostate cancer; Bone marrow derived cells; N-methyl nitrosourea