缺氧(环境)
一氧化氮
内分泌学
内科学
内皮
一氧化氮合酶
精氨酸
血管舒张
内皮功能障碍
化学
医学
氧气
生物化学
有机化学
氨基酸
作者
Yan-Xia Zhao,Tong Li,Guangmei Zhang,Xie-Hui Zhao,Yu-Ping Sa,Yan Liu,Dianxiang Lu,Qin Ga,Ping Wu
标识
DOI:10.1016/j.wem.2020.06.010
摘要
IntroductionOur previous study showed that high-fat diet inhibited the increase in nitric oxide and endothelial nitric oxide synthase expression in the aortic endothelium of rats exposed to hypoxia, and hypoxia plus a high-fat diet led to earlier and more severe vascular endothelial dysfunction (VED) than hypoxia alone. The purpose of the present study was to investigate the effects of L-arginine on high-fat diet–induced VED of rats in hypoxia.MethodsForty male Sprague-Dawley rats were randomly divided into 4 groups and treated with hypoxia (H group), hypoxia plus high-fat diet (H+HFD group), hypoxia plus L-arginine (H+L-Arg group), and hypoxia plus high-fat diet and L-arginine (H+HFD+L-Arg group) for 1 wk. Hypoxia was simulated in a hypobaric chamber with an altitude of 5000 m. Aortic morphology and endothelium-dependent vasorelaxation were used to assess VED.ResultsHigh-fat diet impaired vascular remodeling and reduced endothelium-dependent vasodilator response to acetylcholine in rats exposed to hypoxia, secondary to dysregulation of the nitric oxide pathway. L-arginine supplementation significantly increased plasma nitrates and nitrites and endothelial nitric oxide synthase mRNA levels and improved ultrastructural changes in aortic endothelium and endothelium-dependent vasodilator response.ConclusionsL-arginine prevents aortic ultrastructural changes and reverses VED induced by high-fat diet in rats exposed to hypoxia, which may have implications for VED induced by high-fat diet in high altitude dwellers.
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