整合子
流动遗传元素
整合酶
生物
转座因子
遗传学
水平基因转移
细菌基因组大小
基因组
基因
质粒
重组酶
基因盒
整合酶
转座酶
细菌遗传学
计算生物学
重组
大肠杆菌
作者
Claire Vit,Céline Loot,José Antonio Escudero,Aleksandra Nivina,Didier Mazel
出处
期刊:Methods in molecular biology
日期:2019-10-05
卷期号:: 189-208
被引量:9
标识
DOI:10.1007/978-1-4939-9877-7_14
摘要
Integrons are genetic elements involved in bacterial adaptation to the environment. Sedentary chromosomal integrons (SCIs) can stockpile and rearrange a myriad of different functions encoded in gene cassettes. Through their association with transposable elements and conjugative plasmids, some SCIs have acquired mobility and are now termed Mobile Integrons (MIs). MIs have reached the hospitals and are involved in the rise and spread of antibiotic resistance genes through horizontal gene transfer among numerous bacterial species. Here we aimed at describing methods for the detection of integrons in sequenced bacterial genomes as well as for the experimental characterization of the activity of their different components: the integrase and the recombination sites.
科研通智能强力驱动
Strongly Powered by AbleSci AI