Postischemic Cerebrovascular E-Selectin Expression Mediates Tissue Injury in Murine Stroke

医学 缺血 神经保护 髓过氧化物酶 选择素 冲程(发动机) 生理盐水 麻醉 脑血流 促炎细胞因子 脑缺血 炎症 内科学 机械工程 工程类
作者
Judy Huang,Tanvir F. Choudhri,Christopher J. Winfree,Ryan McTaggart,Szilárd Kiss,J Mocco,Louis J. Kim,Themistocles S. Protopsaltis,Yuan Zhang,David J. Pinsky,E. Sander Connolly
出处
期刊:Stroke [Ovid Technologies (Wolters Kluwer)]
卷期号:31 (12): 3047-3053 被引量:150
标识
DOI:10.1161/01.str.31.12.3047
摘要

Although the deleterious role of several proinflammatory mediators, including P-selectin, in reperfused stroke is well established, the role of E-selectin has not been fully characterized.E-selectin mRNA expression was studied at 4, 10, and 24 hours after reperfusion with reverse transcription and polymerase chain reaction in mice (n=18) subjected to transient intraluminal middle cerebral artery occlusion (MCAO). Mice received intravenous injection with anti-E-selectin monoclonal antibody (10, 35, or 50 microg), nonimmune IgG, or vehicle immediately before MCAO and 90 minutes later (n=85). Others received anti-E-selectin antibody 3 or 6 hours after MCAO (n=32). Myeloperoxidase activity was measured in sham-operated mice and after 10 hours of reperfusion in saline-, nonimmune IgG-, or anti-E-selectin IgG-treated cohorts (n=17). Serial cerebral blood flow was measured with laser-Doppler flowmetry, and outcomes were assessed by neurological deficits and infarct volumes with the use of planimetric analysis of triphenyltetrazolium chloride-stained sections.Upregulated E-selectin expression occurred in the ischemic cerebral vasculature within 4 hours of reperfusion and persisted for 24 hours. Anti-E-selectin antibody increased ischemic cortical cerebral blood flow up to 2.6-fold (P:<0.05). In addition to dose-dependent reductions in neurological deficits (P:<0.05), mortality, and infarct volumes (P:<0.01 for 35 and 50 microg), anti-E-selectin treatment reduced cerebral neutrophil accumulation (P:<0.05) and was neuroprotective even if delayed until 3 hours after ischemia (P:<0. 05).These findings establish a functional role for E-selectin in the pathogenesis of tissue injury after cerebral ischemia and reperfusion and suggest that E-selectin blockade may be clinically useful in the treatment of reperfused stroke.
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