细胞生物学
心肌细胞
组织因子
细胞生长
化学
生物
内科学
医学
生物化学
凝结
作者
Plinio Cirillo,Paolo Golino,Paolo Calabrò,Gaetano Calı̀,Maurizio Ragni,Salvatore De Rosa,Giovanni Cimmino,Mario Pacileo,Raffaele De Palma,L. A. Forte
出处
期刊:Cardiovascular Research
[Oxford University Press]
日期:2005-07-15
卷期号:68 (1): 47-55
被引量:143
标识
DOI:10.1016/j.cardiores.2005.05.010
摘要
Inflammation plays a pivotal role in atherothrombosis. In addition to being a prognostic marker for major cardiovascular events, recent data indicate that C-reactive protein (CRP) might directly promote atherothrombosis by exerting direct effects on vascular cells. The aim of the present study was to determine whether CRP might affect the prothrombotic and proliferative characteristics of endothelial (ECs) and smooth muscle cells (SMCs).Incubation of ECs and SMCs with CRP resulted in a dose-dependent activation of cell proliferation, which was mediated by activation of the p44/42 MAP Kinase (ERK 1/2) pathway. In addition, CRP also induced tissue factor (TF) expression in both cell types in a dose-dependent fashion, exerting its effect at the transcriptional level, as demonstrated by semiquantitative and by real time PCR. Activation of the transcription factor, NF-kappaB, by CRP was demonstrated by EMSA and by suppression of TF expression by the NF-kappaB inhibitor, pyrrolidine-dithio-carbamate ammonium.These data indicate that CRP exerts direct effects on ECs and SMCs by promoting proliferation and TF expression and support the notion that CRP, besides representing a marker of inflammation, is an effector molecule able to induce a pro-atherothrombotic phenotype in cells of the vessel wall.
科研通智能强力驱动
Strongly Powered by AbleSci AI