Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize

数量性状位点 生物 遗传学 人口 等位基因 RAPD 基因型 近交系 大块分离分析 分子标记 表型 遗传标记 限制性片段长度多态性 特质 基因定位 基因 遗传多样性 染色体 人口学 社会学 计算机科学 程序设计语言
作者
S. A. Quarrie,Vesna Lazić-Jančić,Dragan Kovačević,Andrew Steed,S. Pekić
出处
期刊:Journal of Experimental Botany [Oxford University Press]
卷期号:50 (337): 1299-1306 被引量:152
标识
DOI:10.1093/jxb/50.337.1299
摘要

The usual method to locate and compare loci regulating quantitative traits (QTLs) requires a segregating population of plants with each one genotyped with molecular markers. However, plants from such segregating populations can also be grouped according to phenotypic expression of a trait and tested for differences in allele frequency between the population bulks: bulk segregant analysis (BSA). The same probes used for making a genetic map (e.g. isozyme, RFLP, RAPD, etc) can be used for BSA. A molecular marker showing polymorphism between the parents of the population and which is closely-linked to a major QTL regulating a particular trait will mainly co-segregate with that QTL, i.e. segregate according to the phenotype if the QTL has a large effect. Thus, if plants are grouped according to expression of the trait and extreme groups tested with that polymorphic marker, the frequency of the two marker alleles present within each of the two bulks should deviate significantly from the ratio of 151 expected for most populations. As chromosomal locations of many molecular markers have now been determined in many species, the map location of closely-linked QTLs can therefore be deduced without having to genotype every individual in a segregating population. This has been used successfully with composite populations of maize to locate QTLs associated with yield under severe drought. An inbred line derived from one of the populations selected for higher drought yield has been crossed with a drought-susceptible inbred line to produce a mapping population for QTL analysis of physiological and developmental traits likely to regulate yield under drought. Future work to identify traits having QTLs with flanking markers showing significant allele frequency differences in the BSA studies will indicate those traits likely to be important in determining yield under drought.

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