Effects of AKT2 expression on gemcitabine sensitivity in pancreatic cancer cell line Panc-1

Objective To explore the effects of AKT2 expression in pancreatic cancer cell line Panc-1 on the sensitivity towards to gemcitabine. Methods The expression vector pAKT2-shRNA was constructed and transfected into Panc-1 cells by lipofecton. The mRNA and protein expression levels of AKT2 were detected by RT-PCR and Western blot assays, respectively. The changes of gem- citabine sensitivity after shRNA were examined by the MTT assay. Results The mRNA and protein levels of AKT2 in Panc-1 cells were significantly decreased after the transfection, and the median inhi- bition concentration of gemcitabine against Panc-1 cells was reduced from （1.96±0.22） mg/L to （0.24±0.03） mg/L. The sensitivity of Panc-1 cells to gemcitabine was increased significantly after pAKT2-shRNA transfeetion. Conclusion The sensitivity of Panc-1 cells to gemcitabine could be en- hanced by pAKT2-shRNA. Key words: Pancreatic cancer;  Gene;  Gemcitabine