微流控
纤维
材料科学
光纤
环介导等温扩增
炸薯条
制作
检出限
微流控芯片
实验室晶片
纳米技术
荧光
光电子学
光流学
计算机科学
光学
色谱法
化学
物理
电信
病理
复合材料
医学
生物化学
替代医学
DNA
作者
Helen E. Parker,Sanghamitra Sengupta,Achar V. Harish,Inês F. Pinto,Haakan N. Joensson,Walter Margulis,Aman Russom,Fredrik Laurell
摘要
In this work, we present the design and fabrication of a fiber device that performs digital droplet microfluidics for molecular diagnostics. A variety of fibers and capillaries were used to build three connected modules dedicated to droplet generation, incubation, and fluorescence detection which enables a uniaxial arrangement. This is in contrast to the traditional 2-dimensional lab-on-a-chip architecture. We characterize our fiber device using a fluorescein dilution series. Our observed detection limit is on the order of 10 nM fluorescein. We demonstrate our all-fiber device for the fluorescence readout after loop-mediated isothermal amplification (LAMP) of synthetic SARS-CoV-2. Our results suggest that this fiber device can successfully distinguish between positive and negative samples in molecular diagnostics. We propose that our fiber device offers benefits over microfluidic chip techniques such as easier optical integration, much simpler sample loading, and faster diagnosis with high specificity and sensitivity.
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