MP07-07 PRELIMINARY STUDY ON THE ROLE OF SELECTIVE REGULATION OF CaSR-CLAUDIN-14 PATHWAY IN THE FORMATION OF CALCIUM OXALATE KIDNEY STONES IN RATS

You have accessJournal of UrologyStone Disease: Basic Research & Pathophysiology (MP07)1 Sep 2021MP07-07 PRELIMINARY STUDY ON THE ROLE OF SELECTIVE REGULATION OF CaSR-CLAUDIN-14 PATHWAY IN THE FORMATION OF CALCIUM OXALATE KIDNEY STONES IN RATS Biao Qian, Zhiqiang Hao, Jingshen Wang, Xiaofeng Zou, Guoxi Zhang, and Heng Yang Biao QianBiao Qian More articles by this author , Zhiqiang HaoZhiqiang Hao More articles by this author , Jingshen WangJingshen Wang More articles by this author , Xiaofeng ZouXiaofeng Zou More articles by this author , Guoxi ZhangGuoxi Zhang More articles by this author , and Heng YangHeng Yang More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000001980.07AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Through selectively regulating the calcium sensitive receptor-tight junction protein 14 (CaSR-Claudin-14) pathway, this study preliminarily explore the role of this protein pathway in the formation of calcium oxalate kidney stones in rats. METHODS: 120 Wistar male rats were selected and randomly divided into blank control group (NC group), ethylene glycol (EG group), agonist group (EG+ group) and inhibitor group (EG- group), with 30 rats in each group. After modeling, 3 rats were randomly selected from each group and sacrificed every week. Kidney tissue was taken to observe the pathological changes and detect the expression of CaSR and Claudin-14 by Immunofluorescence and Western blot. RESULTS: Except NC group, crystal was found in the pathological smears of the other groups. The EG+ group had the most crystals, followed by the EG group, and the EG- group had the least; Western blot and immunofluorescence results showed that CaSR and Claudin-14 were expressed in the four groups. From the third week, the expression levels of CaSR and Claudin-14 in the remaining three groups gradually decreased except NC group. By the eighth week, the difference in expression of CaSR and Claudin-14 reached the maximum. The expression level from low to high were EG+ group, EG group, EG- group, NC group respectively, and the difference between each group was statistically significant. CONCLUSIONS: The expression of CaSR is negatively correlated with the amount of crystals. The change of Claudin-14 is positively correlated with CaSR. The CaSR-Claudin-14 pathway plays a role in inhibiting the formation of stones. Source of Funding: None © 2021 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 206Issue Supplement 3September 2021Page: e141-e142 Advertisement Copyright & Permissions© 2021 by American Urological Association Education and Research, Inc.MetricsAuthor Information Biao Qian More articles by this author Zhiqiang Hao More articles by this author Jingshen Wang More articles by this author Xiaofeng Zou More articles by this author Guoxi Zhang More articles by this author Heng Yang More articles by this author Expand All Advertisement Loading ...