Accumulation of neutral lipids and carotenoids of Rhodotorula diobovata and Rhodosporidium babjevae cultivated under nitrogen-limited conditions with glycerol as a sole carbon source

ABSTRACT A total of two red oleaginous yeasts, Rhodotorula diobovata and Rhodosporidium babjevae, were investigated for their potential to grow on nitrogen-limited media with sufficient glycerol as carbon source and produce biomass, triacylglycerides (TAGs) and carotenoids. The two yeasts produced equal quantities of biomass by 120 h post-inoculation (h pi), but R. diobovata consumed more glycerol than R. babajavae under the same conditions. The TAG concentrations accumulated by R. diobovata and R. babjevae were greater than 20% dry cell weight (dcw), and the major fatty acid components consisted of palmitic acid, oleic acid and linolenic acid. The highest concentration of total fatty acids in biomass were present during the late of stationary phase were 486.3 mg/g dcw for R. diobovata at 120 h pi, and 243.9 mg/g dcw for R. babjevae at 144 h pi. Both R. diobovata and R. babjevae produced high concentrations of torularhodin, and low amounts of torulene and γ-carotene. Total carotenoid concentrations in R. diobovata biomass were 31.5 mg/g dcw at 120 h pi and 43.1 mg/g dcw at 96 h pi for R. babjevae. The dcw accumulations of carotenoids by R. diobovata and R. babjevae were significantly greater than those reported for other carotenogenic Rhodotorula and Rhodosporidium strains.